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Neb Polymerase Chart

Neb Polymerase Chart - Error rates represent base substitution errors only and are reported as the number of. Dna is a dynamic molecule whose structure is stabilized by a large number of weak interactions. Web use the neb tm calculator to estimate an appropriate annealing temperature when using neb pcr products. T7 and sp6 exhibit high specificity for their respective promoters. The following table lists properties that should be considered when choosing a polymerase. Web t4 dna polymerase catalyzes the synthesis of dna in the 5´→ 3´ direction and requires the presence of template and primer. Select the polymerase or kit from the list of products. T7 and sp6 rna polymerases are dna dependent rna polymerases that produce dna templated rna transcripts. Select the product group of the polymerase or kit you plan to use. Web taq dna polymerase is a thermostable dna polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).

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This Enzyme Has A 3´→ 5´ Exonuclease Activity Which Is Much More Active Than That Found In Dna Polymerase I ( E.

Web at ~280x higher than taq, q5 offers unparalleled fidelity for your most important samples. Web taq dna polymerase is a thermostable dna polymerase that possesses a 5´→3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). If needed, modify the recommended primer concentration. Web rna polymerase selection chart.

Select The Polymerase Or Kit From The List Of Products.

Dna is a dynamic molecule whose structure is stabilized by a large number of weak interactions. Web neb’s restriction enzyme buffer system makes your restriction digests easy and convenient. Recommended hiscribe rna synthesis kits by application; We are able to offer >210 restriction enzymes that cut in a single buffer, rcutsmart™.

Web Use The Neb Tm Calculator To Estimate An Appropriate Annealing Temperature When Using Neb Pcr Products.

The 99 kd enzyme catalyzes in vitro rna synthesis from a cloned dna sequence under the t7 promoters. It is supplied with 10x thermopol reaction buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations. Web t4 dna polymerase catalyzes the synthesis of dna in the 5´→ 3´ direction and requires the presence of template and primer. Several factors govern which polymerase should be used in a given application, including:

Select The Product Group Of The Polymerase Or Kit You Plan To Use.

Since these properties can depend on reaction conditions, the primary references should be consulted prior to use in a given application. T7 and sp6 exhibit high specificity for their respective promoters. Web neb's dna polymerase selection chart will assist in choosing a polymerase for your application. Select the product group of the polymerase or kit you plan to use.

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